Haploidization Analysis in Penicillium chrysogenum
نویسندگان
چکیده
منابع مشابه
Penicillin acyltransferase in Penicillium chrysogenum.
Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase was purified threefold by adsorption on calcium phosphate gel at pH 6 and elutio...
متن کاملAnalysis of Vegetative Segregation and Recombination in Penicillium Chrysogenum.
N the basis of an analysis of vegetative segregation in heterozygous diploids of 0 Aspergillus nidulans, PONTECORVO et al. (PONTECORVO 1953 ; PONTECORVO, TARR GLOOR and FORBES 1954) concluded that the segregant nuclei arise as a consequence of two independent processes. The first is mitotic crossing over (STERN 1936), giving rise to diploid nuclei which are homozygous for some of the markers. T...
متن کاملThe lactic dehydrogenase of Penicillium chrysogenum.
A soluble, cytochrome linked flavoprotein which oxidizes lactic acid to pyruvic acid has been isolated from Penicillium chrysogenum, strain NRRL 1951 -B25. The reduction of cytochrome c by a flavin enzyme has precedence in the work of Horecker and Heppel (1949) and Mlorell (1952) who have shown that xanthine oxidase can reduce cytochrome c. Morell reported the enzyme to be inhibited strongly by...
متن کاملBallistic disruption of Penicillium chrysogenum cells.
The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in...
متن کاملEffects of lysine analogs on Penicillium chrysogenum.
Compounds structurally related to lysine were tested against Penicillium chrysogenum Wis. 54-1255 for inhibition of growth, sporulation, and penicillin formation. This strain is relatively resistant to lysine analogs. The compounds that were the more active inhibitors of growth and whose activities were reversed by L-lysine were diaminohexynoic acid, N-epsilon-methyllysine, N-alpha-methyllysine...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1971
ISSN: 0022-1287
DOI: 10.1099/00221287-66-1-63